anti-VIM antibody product blog
Tags: Antibody; VIM; Polyclonal Antibody; anti-VIM antibody; Vimentin (VIM);
The VIM vim (Catalog #MBS2006603) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Vimentin (VIM) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Vimentin (VIM) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB).Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VIM vim product has the following accession number(s) (GI #62414289) (NCBI Accession #NP_003371.2) (Uniprot Accession #P08670). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-STRSVS SSS YRRMFGGPGT ASRPSSSRSY VTTSTRTYSL GSALRPSTSR SLYASSPGGV YATRSSAVRL RSSVPGVRLL QDSVDFSLAD AINTEFKNTR TNEKVELQEL NDRFANYIDK VRFLEQQNKI LLAELEQLKG QGKSRLGDLY EEEMRELRRQ VDQLTNDKAR VEVERDNLAE DIMRLREKLQ EEMLQREEAE NTLQSFRQDV DNASLARLDL ERKVESLQEE IAFLKKLHEE EIQELQAQIQ EQHVQIDVDV SKPDLTAALR DVRQQYESVA AKNLQEAEEW YKSKFADLSE AANRNNDALR QAKQESTEYR RQVQSLTCEV DALKGTNESL ERQMREMEEN FAVEAANYQD TIGRLQDEIQ NMKEEMARHL REYQDLLNVK MALDIEIATY RKLLEGEESR ISLPLPNFSS LNLRETNLDS LPLVDTHSKR TLLIKTVETR DGQVINETSQ HHDDLE.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Vimentin (VIM) with the following immunoassay(s):
Western Blot (WB) (Western Blot: Sample: Lane1: Human 293T Cells; Lane2: Human Hela Cells; Lane3: Rat Heart Tissue; Lane4: Mouse Heart Tissue.)
Western Blot (WB) (Western Blot: Sample: Recombinant VIM, Human.)
Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded lung tissue))
Immunohistochemistry (IHC) (FITC staining on IHC-P Simple: Mcf7 cells))
Immunogen: Recombinant VIM (Ser2~Glu466) expressed in E.coli.
Cross Reactivity: Human, Mouse, Rat. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2050859. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VIM are readily searchable from our website. Different antibodies against the same target such as VIM may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Bone Marrow, Brain, Eye, Kidney, Liver, Muscle, Nerve, Vascular tissues are correlated with this protein.