anti-VIM antibody product blog
Tags: Antibody; Vimentin; VIM; Polyclonal Antibody; anti-VIM antibody;
The VIM vim (Catalog #MBS9211324) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Vimentin Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Hamster, Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. MyBioSource\'s Vimentin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).WB~~1:1000. Researchers should empirically determine the suitability of the VIM vim for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VIM vim product has the following accession number(s) (GI #62414289) (NCBI Accession #NP_003371.2) (Uniprot Accession #P08670). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Vimentin Antibody (Center) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Vimentin Antibody (Center) in A2058,A375 cell line lysates (35ug/lane). Vimentin (arrow) was detected using the purified Pab.)
Western Blot (WB) (Western blot analysis of Vimentin (arrow) using Vimentin Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC) (Vimentin Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Vimentin Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (Vimentin Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VIM are readily searchable from our website. Different antibodies against the same target such as VIM may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.