anti-VRK1 antibody product blog
Tags: Antibody; Polyclonal Antibody; Vaccinia Related Kinase 1; VRK1; anti-VRK1 antibody;
The VRK1 vrk1 (Catalog #MBS625783) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Vaccinia Related Kinase 1 (VRK1, Serine/threonine-protein kinase VRK1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Vaccinia Related Kinase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500. Researchers should empirically determine the suitability of the VRK1 vrk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VRK1 vrk1 product has the following accession number(s) (GI #109730507) (Uniprot Accession #Q99986). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Vaccinia Related Kinase 1 (VRK1, Serine/threonine-protein kinase VRK1) with the following immunoassay(s):
Western Blot (WB) (The anti-VRK1 Pab is used in Western blot to detect VRK1 in mouse lung tissue lysate (Lane 1) and HL-60 cell lysate (Lane 2).)
Western Blot (WB) (Western blot analysis of VRK1 (arrow) using rabbit polyclonal VRK1 Antibody (E381). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VRK1 gene.)
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.
Immunogen: Synthetic peptide selected from the center region of human VRK1 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VRK1 are readily searchable from our website. Different antibodies against the same target such as VRK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.