anti-WAS antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-WAS antibody; WAS;
The WAS was (Catalog #MBS9414112) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WAS Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s WAS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100. Researchers should empirically determine the suitability of the WAS was for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The WAS was product has the following accession number(s) (GI #4507909) (NCBI Accession #NP_000368.1) (Uniprot Accession #P42768). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the WAS Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using WAS antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human esophagus using WAS antibody at dilution of 1:100 (200x lens).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat kidney using WAS antibody at dilution of 1:100 (200x lens).)
Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8).
Immunogen Type: Recombinant Protein
Immunogen Description: Recombinant protein of human WAS. Target Name: WAS. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing WAS are readily searchable from our website. Different antibodies against the same target such as WAS may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.