anti-WEE1 antibody product blog
Tags: Antibody; Polyclonal Antibody; Wee1; anti-Wee1 antibody;
The WEE1 wee1 (Catalog #MBS9407093) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WEE1 (Phospho-Ser642) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s WEE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Western blotting: 1:500~1:1000. Researchers should empirically determine the suitability of the WEE1 wee1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The WEE1 wee1 product has the following accession number(s) (GI #221307502) (NCBI Accession #NP_001137448.1) (Uniprot Accession #P30291). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the WEE1 (Phospho-Ser642) Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from 293 cells treated with etoposide using WEE1 (Phospho-Ser642) Antibody.The lane on the right is treated with the antigen-specific peptide.)
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on \'Tyr-15\'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on \'Tyr-15\' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.Watanabe N., EMBO J. 14:1878-1891(1995).Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001).Igarashi M., Nature 353:80-83(1991).
Immunogen Type: Peptide-KLH
Immunogen Description: Peptide sequence around phosphorylation site of Serine 642(S-V-S(p)-L-T) derived from Human WEE1. Modification: Phospho-Ser642
Target Name: WEE1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing WEE1 are readily searchable from our website. Different antibodies against the same target such as WEE1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. WEE1 also interacts with the following gene(s): CCNB1, CDC25A, CDC25B, CDC25C, CDK1, CDK2, HSP90AA1, PLK1.