anti-Wnt-3A antibody product blog
Tags: Antibody; Polyclonal Antibody; Wnt-3a; anti-Wnt-3a antibody;
The Wnt-3A wnt3a (Catalog #MBS691145) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human Wnt-3A reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Wnt-3A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).Western Blot: To detect human WNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human WNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Researchers should empirically determine the suitability of the Wnt-3A wnt3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Wnt-3A wnt3a product has the following accession number(s) (GI #14916475) (NCBI Accession #NP_149122.1) (Uniprot Accession #P56704). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. During development, Wnt-3a is a morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination. When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation. Wnt-3a has also been implicated in chondrocyte differentiation. Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity. A second site at Ser 209 modified by palmitoleic acid also contributes. Human Wnt-3a shares 96% amino acid (aa) identity with mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt-3. Human Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues.
Antibody Generation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human WNT-3a. Anti-Human WNT-3a specific antibody was purified by affinity chromatography employing immobilized human WNT-3a matrix. Buffer: PBS; pH 7.2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Wnt-3A are readily searchable from our website. Different antibodies against the same target such as Wnt-3A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.