anti-WNT4 antibody product blog
Tags: Antibody; WNT4; Polyclonal Antibody; anti-WNT4 antibody;
The WNT4 wnt4 (Catalog #MBS626947) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Wnt4, CT (Protein Wnt-4, UNQ426/PRO864) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Wnt4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the WNT4 wnt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The WNT4 wnt4 product has the following accession number(s) (GI #13430197) (Uniprot Accession #P56705). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Wnt4, CT (Protein Wnt-4, UNQ426/PRO864) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of WNT4 antibody (C-term) in 293 cell line lysates (35ug/lane). WNT4 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) ((LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma reacted with WNT4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of 293 cells using WNT4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
The WNT family consists of secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT4 is a protein which shows 98% amino acid identity to the Wnt4 protein of mouse and rat. It play a concerted role in both the control of female development and the prevention of testes formation.
Immunogen: Synthetic peptide selected from the C-terminal region of human WNT4 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing WNT4 are readily searchable from our website. Different antibodies against the same target such as WNT4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain, Embryonic Tissue, Eye, Heart, Kidney, Lung, Mammary Gland, Ovary, Placenta, Uterus tissues are correlated with this protein.