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anti-XRCC6 antibody product blog

Posted on 2015-09-23 12:40:25 by mybiosource_staff
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Tags: Antibody; XRCC6; anti-XRCC6 antibody; Polyclonal Antibody;
The XRCC6 xrcc6 (Catalog #MBS9203893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The XRCC6 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s XRCC6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB).
WB~~1:1000. Researchers should empirically determine the suitability of the XRCC6 xrcc6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The XRCC6 xrcc6 product has the following accession number(s) (GI #4503841) (NCBI Accession #NP_001460.1) (Uniprot Accession #P12956). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the XRCC6 Antibody (Center) with the following immunoassay(s):
Western Blot (WB) (XRCC6 Antibody (Center) western blot analysis in 293,A549 cell line lysates (35ug/lane).This demonstrates the XRCC6 antibody detected the XRCC6 protein (arrow).)
Western Blot (WB) XRCC6.

Immunohistochemistry (IHC) (XRCC6 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XRCC6 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC) XRCC6.

Flow Cytometry (FC/FACS) (XRCC6 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS) XRCC6.

Immunofluorescence (IF) (Confocal immunofluorescent analysis of XRCC6 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF) XRCC6.

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus.

Antigen Type: Synthetic Peptide
Crown Antibody: Yes. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing XRCC6 are readily searchable from our website. Different antibodies against the same target such as XRCC6 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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