anti-YARS antibody product blog
Tags: Antibody; YARS; anti-YARS antibody; Polyclonal Antibody;
The YARS yars (Catalog #MBS9126424) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The YARS Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s YARS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the YARS yars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The YARS yars product has the following accession number(s) (GI #13638438) (NCBI Accession #P54577.4) (Uniprot Accession #P54577). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the YARS Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using YARS antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.)
Immunoprecipitation (IP) (Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug YARS antibody. Western blot was performed from the immunoprecipitate using YARS antibody at a dilition of 1:1000.)
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human YARS
Calculated Molecular Weight: 59kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing YARS are readily searchable from our website. Different antibodies against the same target such as YARS may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.