|The YBX1 ybx1 (Catalog #MBS9128372) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The YBX1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s YBX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
WB 1:500 - 1:2000
IHC 1:50 - 1:200. Researchers should empirically determine the suitability of the YBX1 ybx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The YBX1 ybx1 product has the following accession number(s) (GI #34098946) (NCBI Accession #NP_004550.2) (Uniprot Accession #P67809). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the YBX1 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using YBX1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung cancer using YBX1 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate using YBX1 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse brain using YBX1 antibody at dilution of 1:100 (40x lens).)
Route: Synthetic Peptide. Immunogen: A synthetic peptide of human YBX1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing YBX1 are readily searchable from our website. Different antibodies against the same target such as YBX1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Kidney, Liver, Lung, Muscle, Prostate, Skin, Thyroid, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. Breast Neoplasms, Carcinoma, Hepatocellular, Disease Models, Animal, Immune System Diseases, Kidney Diseases, Liver Diseases, Liver Neoplasms, Lung Diseases, Neoplasms, Experimental, Ovarian Neoplasms are some of the diseases may be linked to YBX1 Polyclonal Antibody. YBX1 also interacts with the following gene(s): DHX9, HNRNPA2B1, HNRNPD, HNRNPU, PTPN1, TP53, UBC.