anti-ZEB2 antibody product blog
Tags: Antibody; Polyclonal Antibody; ZEB2; anti-ZEB2 antibody; Smad Interacting Protein 1;
The ZEB2 zeb2 (Catalog #MBS176211) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Smad Interacting Protein 1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Smad Interacting Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the ZEB2 zeb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The ZEB2 zeb2 product has the following accession number(s) (GI #13124503) (NCBI Accession #O60315.1) (Uniprot Accession #O60315). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Smad Interacting Protein 1 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-ZEB2 antibody, MBS176211, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: HELA Cell Lysate
)
Description: Rabbit IgG polyclonal antibody for Zinc finger E-box-binding homeobox 2(ZEB2) detection. Tested with WB in Human, Mouse, Rat.
Background: ZEB2(Zinc finger E-box-binding homeobox2), also known as SIP1or ZINC FINGER HOMEOBOX 1B(ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The ZEB2 gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs, the transducers of TGF-beta signaling, and interacts with the nucleosome remodeling and histone deacetylation(NURD) complex. By radiation hybrid analysis, Nagase et al.(1998) mapped the ZEB2 gene to chromosome 2. Wakamatsu et al.(2001) mapped the ZEB2 gene to chromosome 2q22. Vandewalle et al.(2005) showed that expression of mouse Sip1 in human epithelial cells caused a morphologic change from an epithelial to a mesenchymal phenotype. Expression of SNAI1 in epithelial cells triggers an epithelial-mesenchyme transition. Beltran et al.(2008) showed that synthesis of ZEB2 was upregulated following SNAI1 expression in human cell lines.
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Smad Interacting Protein 1 (948-962aa DMQQRRKYQRKQGFQ), identical to the related rat and mouse sequences. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ZEB2 are readily searchable from our website. Different antibodies against the same target such as ZEB2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.