anti-a1ACT antibody product blogThe a1ACT serpina3 (Catalog #MBS2003816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Alpha-1-Antichymotrypsin (a1ACT) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Alpha-1-Antichymotrypsin (a1ACT) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB).
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the a1ACT serpina3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The a1ACT serpina3 product has the following accession number(s) (GI #50659080) (NCBI Accession #NP_001076.2) (Uniprot Accession #P01011). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -KKKWVM VPMMSLHHLT IPYFRDEELS CTVVELKYTG NASALFILPD QDKMEEVEAM LLPETLKRWR DSLEFREIGE LYLPKFSISR DYNLNDILLQ LGIEEAFTSK ADLSGITGAR NLAVSQVVHK AVLDVFEEGT EASAATAVKI TLLSALVETR TIVR.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Alpha-1-Antichymotrypsin (a1ACT) with the following immunoassay(s):
Western Blot (WB) ((Figure. Western Blot; Sample: Recombinant a1ACT, Human.))
Immunohistochemistry (IHC) ((Figure.DAB staining on IHC-P. Samples: Human Tissue))
Immunogen: Recombinant a1ACT (Lys235~Arg394) expressed in E.coli. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2050478. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing a1ACT are readily searchable from our website. Different antibodies against the same target such as a1ACT may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain, Liver, Lung, Uterus tissues are correlated with this protein. a1ACT also interacts with the following gene(s): APP, CTSG, MMP1. The following patways have been known to be associated with this gene.