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anti-ATP5A1 antibody product blog

Tags: Antibody; Polyclonal Antibody; anti-ATP5A1 antibody; ATP5A1;

     The ATP5A1 atp5a1 (Catalog #MBS7047729) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP5A1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s ATP5A1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP).
WB: 1: 1000-1: 5000
IHC: 1: 20-1: 200
IP: 1: 200-1: 2000. Researchers should empirically determine the suitability of the ATP5A1 atp5a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The ATP5A1 atp5a1 product has the following accession number(s) (GI #50345982) (NCBI Accession #NP_001001935.1) (Uniprot Accession #P25705). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ATP5A1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot
Positive WB detected in:Hela whole cell lysate,K562 whole cell lysate,MCF-7 whole cell lysate,PC3 whole cell lysate,Mouse heart tissue
All lanes: ATP5A1 antibody at 2.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60,55,58 kDa
Observed band size: 60,58 kDa
)
Western Blot (WB) ATP5A1.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer using MBS7047729 at dilution of 1:100)
Immunohistochemistry (IHC) ATP5A1.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7047729 at dilution of 1:100)
Immunohistochemistry (IHC) ATP5A1.

Immunoprecipitation (IP) (Immunoprecipitating ATP5A1 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG(1ug)instead of MBS7047729 in Hela whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)
Lane 2: MBS7047729(4ug)+ Hela whole cell lysate(500ug)
Lane 3: Hela whole cell lysate (20ug)
)
Immunoprecipitation (IP) ATP5A1.

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).

Immunogen: Recombinant Human ATP synthase subunit alpha, mitochondrial protein (44-257AA).
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