anti-HIV-1 antibody product blogThe HIV-1 n/a (Catalog #MBS324131) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s HIV-1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence.
Titer: >1:1,000 by indirect immunofluorescence. Researchers should empirically determine the suitability of the HIV-1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/ml) as a stabilizer and 0.1% sodium azide preservative. Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. ViroStat also recommends use of its COUNTERSTAIN/BLOCKING DILUENT (Cat.#4400) and its MOUNTING MEDIUM (Cat.#4300) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing ViroStat\'s enzyme-antibody conjugates specific to FITC (Cat.#s 3104 & 3105). Recommened short term (<6 months) storage is liquid at 2-8 degree C under subdued light. For longer term storage, aliquot and freeze. Immunoglobulin concentration is 4-5 mg/ml. Unit size is 1.0 ml.
Immunogen: human isolate, confirmed
Uninfected Cell Reactivity: Does not react with T4+ human lymphocytes. Antiserum Characterization: (neat). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing HIV-1 are readily searchable from our website. Different antibodies against the same target such as HIV-1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.