anti-PAG1 antibody product blogThe PAG1 pag1 (Catalog #MBS2027903) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Phosphoprotein Associated With Glycosphingolipid Microdomains 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA).
Western blotting: 0.2-2ug/mL;1:250-2500
Optimal working dilutions must be determined by end user.
. Researchers should empirically determine the suitability of the PAG1 pag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PAG1 pag1 product has the following accession number(s) (GI #11560063) (NCBI Accession #NP_071589.1) (Uniprot Accession #Q9JM80). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- IPP ENAVDAMLTA RAADGDSGPG VEGPYEVLKD SSSQENMVED CLYETVKEIK EVADKSQGGK SKSTSALKEL QGAHAEGKAD FAEYASVDRN KKCRHSTNAE SILGTSSDLD EETPPPVPVK LLDENANLPE KGEHGAEEQA PEAPSGHSKR FSSLSYKSRE EDPTLTEEEI SAMYSSVNKP GQSAHKPGPE.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1) with the following immunoassay(s):
Western Blot (WB) (Western Blot: Sample: Rat Serum; Primary Ab: 3ug/ml Rabbit Anti-Rat PAG1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB) (Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded testis tissue))
Fragment: PAG1 (Ile138~Phe357)
Organism Species: Rattus norvegicus (Rat). Conjugate: No Conjugate
Immunogen: Recombinant PAG1 (Ile138~Phe357) expressed in E Coli.
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2078349. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PAG1 are readily searchable from our website. Different antibodies against the same target such as PAG1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Breast Neoplasms, Hypertension, Inflammation, Leukemia, Leukemia, Myeloid, Acute, Lung Neoplasms, Lymphoma, Neoplasms, Experimental, Prostatic Neoplasms, Retinoblastoma are some of the diseases may be linked to Polyclonal Antibody to Phosphoprotein Associated With Glycosphingolipid Microdomains 1 (PAG1).