anti-SMAD4 antibody product blogThe Smad4 smad4 (Catalog #MBS540518) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Smad4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Smad4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB).
Dot Blot: 1:10,000
Western Blot: 1:500. Researchers should empirically determine the suitability of the Smad4 smad4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Smad4 smad4 product has the following accession number(s) (GI #4885457) (NCBI Accession #NP_005350.1) (Uniprot Accession #Q13485). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Smad4 Antibody with the following immunoassay(s):
Western Blot (WB)
Affinity Purified Mothers against decapentaplegic homolog 4 Antibody N-epitope
In muscle physiology, plays a central role in balance between atrophy and hypertrophy. May act as a tumor suppressor.
Immunogen: Synthetic peptide taken within amino acid region 131-181 on human SMAD4 protein.
Immunoglobulin: IgG. Determinant: N-epitope
Molecular Function: Chromatin binding, core promoter proximal region sequence-specific DNA binding, identical protein binding I-SMAD binding, metal ion binding
Structure: Found in a complex with SMAD1 and YY1 (By similarity). Interacts with CITED2 (By similarity). Monomer; in the absence of TGF-beta activation. Heterodimer; on TGF-beta activation. Composed of two molecules of a C-terminally phosphorylated R-SMAD molecule, SMAD2 or SMAD3, and one molecule of SMAD4 to form the transcriptional active SMAD2/SMAD3-SMAD4 complex. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with ATF2, COPS5, DACH1, MSG1, SKI, STK11/LKB1, STK11IP and TRIM33. Interacts with ZNF423; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with ZNF521; the interaction takes place in response to BMP2 leading to activation of transcription of BMP target genes. Interacts with USP9X. Interacts (via the MH1 and MH2 domains) with RBPMS. Interacts with WWTR1 (via coiled-coil domain). Component of the multimeric complex SMAD3/SMAD4/JUN/FOS which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Interacts with CITED1. Interacts with PDPK1 (via PH domain) (By similarity). Interacts with VPS39; this interaction affects heterodimer formation with SMAD3, but not with SMAD2, and leads to inhibition of SMAD3-dependent transcription activation. Interactions with VPS39 and SMAD2 may be mutually exclusive.
Subcellular Location: Cytoplasm, Nucleus (Note: Cytoplasmic in the absence of ligand. Migrates to Nucleus when complexed with R-SMAD. PDPK1 prevents its nuclea translocation in response to TGF-beta.